| Monday 26th September 2016 | Premier Division | ||
| 19:45 | Maine Road v Bootle | Brantingham Road | |
| Tuesday 27th September 2016 | Premier Division | ||
| 19:45 | 1874 Northwich v AFC Liverpool | St Luke`s Barton Stadium | |
| 19:45 | Abbey Hey v Barnton | The Abbey Stadium | |
| 19:45 | Ashton Athletic v Winsford United | Brocstedes Park | |
| 19:45 | Barnoldswick Town v Irlam | Silentnight Stadium | |
| 19:45 | Cammell Laird 1907 v Congleton Town | North West Construction Stadium | |
| Tuesday 27th September 2016 | First Division | ||
| 19:45 | Chadderton v Silsden | ARK Fleetech Stadium | |
| 19:45 | City of Liverpool FC v Whitchurch Alport | Delta Taxi Stadium | |
| 20:00 | Stockport Town v Litherland REMYCA | Lambeth Grove | |
| Tuesday 27th September 2016 | Derbyshire Senior Cup 1st Rnd | ||
| 19:45 | Shirebrook Town v New Mills | Shirebrook Staff Sports and Social Club | |
| Tuesday 27th September 2016 | Lancashire Challenge Trophy 1st Rnd | ||
| 19:45 | Bamber Bridge v Charnock Richard | Sir Tom Finney Stadium | |
| Tuesday 27th September 2016 | Manchester Premier Cup 1st Rnd | ||
| 19:45 | Radcliffe Borough v West Didsbury & Chorlton | Stainton Park | |
| Wednesday 28th September 2016 | Premier Division | ||
| 19:45 | Runcorn Town v Hanley Town | Pavilions | |
| Wednesday 28th September 2016 | First Division | ||
| 19:45 | Cheadle Town v Widnes | Park Road Stadium | |
| Wednesday 28th September 2016 | Lancashire Challenge Trophy 1st Rnd | ||
| 20:00 | Bacup Borough v Padiham | Brian Boys West View Stadium | |
| 19:45 | Prestwich Heys v Chorley | Adie Moran Park | |
| Friday 30th September 2016 | Premier Division | ||
| 19:45 | AFC Liverpool v Cammell Laird 1907 | The Marine Travel Arena | |
| Saturday 1st October 2016 | The Macron Cup 1st Rnd | ||
| 15:00 | Barnton v Nelson | The Hinchliffe Holmes Stadium | |
| 15:00 | Carlisle City v Ashton Athletic | Gillford Park Stadium, | |
| 15:00 | Congleton Town v Prestwich Heys | Richborough Estates Stadium | |
| 15:00 | FC Oswestry Town v Irlam | The Venue | |
| 15:00 | Padiham v 1874 Northwich | YESSS Electrical Arena | |
| 15:00 | Runcorn Linnets v Daisy Hill | Millbank Linnets Stadium | |
| 15:00 | Sandbach United v Stockport Town | Sandbach Community Football Centre | |
| 15:00 | Squires Gate v New Mills | The Royal British Legion Stadium | |
| 15:00 | St Helens Town v AFC Darwen | Valerie Park | |
| Played at Prescot Cables | |||
| 15:00 | West Didsbury & Chorlton v Barnoldswick Town | The Recreation Ground | |
| 15:00 | Widnes v Alsager Town | Woodland Court | |
| Played at Alsager Town | |||
| 15:00 | Winsford United v Atherton LR | St Luke`s Barton Stadium | |
| Saturday 1st October 2016 | Premier Division | ||
| 15:00 | Abbey Hey v Hanley Town | The Abbey Stadium | |
| 15:00 | Atherton Collieries v Maine Road | The Kensite Stadium | |
| 15:00 | Bootle v Runcorn Town | Delta Taxi Stadium | |
| Saturday 1st October 2016 | First Division | ||
| 15:00 | Ashton Town v Charnock Richard | The Ashton Town Stadium | |
| 15:00 | Eccleshall v Bacup Borough | Pershall Park | |
| 15:00 | Holker Old Boys v Cheadle Town | Rakesmoor | |
| 15:00 | Litherland REMYCA v AFC Blackpool | Litherland Sports Park | |
| 15:00 | Silsden v City of Liverpool FC | Keighley Road Stadium | |
| 15:00 | Whitchurch Alport v Chadderton | Yockings Park | |
| Monday 3rd October 2016 | First Division | ||
| 19:45 | Daisy Hill v Bacup Borough | New Sirs | |
| Tuesday 4th October 2016 | Premier Division | ||
| 19:45 | 1874 Northwich v Barnton | St Luke`s Barton Stadium | |
| 19:45 | Ashton Athletic v Atherton Collieries | Brocstedes Park | |
| 19:45 | Barnoldswick Town v Padiham | Silentnight Stadium | |
| 19:45 | Congleton Town v Winsford United | Richborough Estates Stadium | |
| 19:45 | Hanley Town v New Mills | Abbey Lane | |
| 19:45 | Runcorn Linnets v Bootle | Millbank Linnets Stadium | |
| 19:45 | West Didsbury & Chorlton v Squires Gate | The Recreation Ground | |
| Tuesday 4th October 2016 | First Division | ||
| 19:45 | AFC Blackpool v Charnock Richard | Mechanics Ground | |
| 19:45 | City of Liverpool FC v FC Oswestry Town | Delta Taxi Stadium | |
| 19:45 | Whitchurch Alport v Widnes | Yockings Park | |
| Tuesday 4th October 2016 | Manchester Premier Cup 1st Rnd | ||
| 19:45 | Abbey Hey v Trafford | The Abbey Stadium | |
| 19:45 | Chadderton v FC United of Manchester | ARK Fleetech Stadium | |
| 19:45 | Glossop North End v Maine Road | Surrey Street | |
| 19:45 | Irlam v Salford City | The Ambitek Stadium | |
| Wednesday 5th October 2016 | Premier Division | ||
| 19:45 | AFC Darwen v Nelson | WEC Group Anchor Ground | |
| 19:45 | Runcorn Town v AFC Liverpool | Pavilions | |
| Wednesday 5th October 2016 | First Division | ||
| 19:45 | Cheadle Town v Alsager Town | Park Road Stadium | |
| Friday 7th October 2016 | Premier Division | ||
| 19:45 | West Didsbury & Chorlton v Irlam | The Recreation Ground | |
| Saturday 8th October 2016 | The Reusch First Division Cup 1st Rnd | ||
| 15:00 | AFC Blackpool v Carlisle City | Mechanics Ground | |
| 15:00 | Bacup Borough v Chadderton | Brian Boys West View Stadium | |
| 15:00 | Eccleshall v Stockport Town | Pershall Park | |
| 15:00 | Holker Old Boys v Ashton Town | Rakesmoor | |
| 15:00 | Prestwich Heys v Litherland REMYCA | Adie Moran Park | |
| 15:00 | Widnes v FC Oswestry Town | Select Security Stadium | |
| Saturday 8th October 2016 | Premier Division | ||
| 15:00 | 1874 Northwich v Abbey Hey | St Luke`s Barton Stadium | |
| 15:00 | AFC Darwen v Cammell Laird 1907 | WEC Group Anchor Ground | |
| 15:00 | Ashton Athletic v Congleton Town | Brocstedes Park | |
| 15:00 | Barnoldswick Town v Bootle | Silentnight Stadium | |
| 15:00 | Barnton v Padiham | The Hinchliffe Holmes Stadium | |
| 15:00 | Hanley Town v Squires Gate | Abbey Lane | |
| 15:00 | Maine Road v Runcorn Linnets | Brantingham Road | |
| 15:00 | Nelson v AFC Liverpool | Little Wembley | |
| 15:00 | New Mills v Atherton Collieries | Church Lane | |
| 15:00 | Runcorn Town v Winsford United | Pavilions | |
| Saturday 8th October 2016 | First Division | ||
| 15:00 | Alsager Town v St Helens Town | Woodland Court | |
| 15:00 | Atherton LR v Whitchurch Alport | Crilly Park | |
| 15:00 | Charnock Richard v Cheadle Town | Mossie Park | |
| 15:00 | City of Liverpool FC v Daisy Hill | Delta Taxi Stadium | |
| 15:00 | Sandbach United v Silsden | Sandbach Community Football Centre | |
| Monday 10th October 2016 | Premier Division | ||
| 19:45 | Atherton Collieries v Nelson | The Kensite Stadium | |
| 19:45 | Winsford United v Maine Road | St Luke`s Barton Stadium | |
| Tuesday 11th October 2016 | Premier Division | ||
| 19:45 | Ashton Athletic v West Didsbury & Chorlton | Brocstedes Park | |
| 19:45 | Barnoldswick Town v Squires Gate | Silentnight Stadium | |
| 19:45 | Bootle v Barnton | Delta Taxi Stadium | |
| 19:45 | Runcorn Linnets v Cammell Laird 1907 | Millbank Linnets Stadium | |
| Tuesday 11th October 2016 | First Division | ||
| 19:45 | AFC Blackpool v St Helens Town | Mechanics Ground | |
| 19:45 | Ashton Town v Atherton LR | The Ashton Town Stadium | |
| 19:45 | Carlisle City v Charnock Richard | Gillford Park Stadium, | |
| 19:45 | Chadderton v Stockport Town | ARK Fleetech Stadium | |
| 19:45 | Sandbach United v Whitchurch Alport | Sandbach Community Football Centre | |
| Wednesday 12th October 2016 | Premier Division | ||
| 19:45 | AFC Liverpool v Abbey Hey | The Marine Travel Arena | |
| 19:45 | Padiham v AFC Darwen | YESSS Electrical Arena | |
| Wednesday 12th October 2016 | First Division | ||
| 19:45 | Litherland REMYCA v City of Liverpool FC | Litherland Sports Park | |
| Saturday 15th October 2016 | Premier Division | ||
| 15:00 | Abbey Hey v Nelson | The Abbey Stadium | |
| 15:00 | AFC Liverpool v New Mills | The Marine Travel Arena | |
| 15:00 | Atherton Collieries v Irlam | The Kensite Stadium | |
| 15:00 | Bootle v Hanley Town | Delta Taxi Stadium | |
| 15:00 | Cammell Laird 1907 v Maine Road | North West Construction Stadium | |
| 15:00 | Congleton Town v AFC Darwen | Richborough Estates Stadium | |
| 15:00 | Padiham v Runcorn Linnets | YESSS Electrical Arena | |
| 15:00 | Runcorn Town v Ashton Athletic | Pavilions | |
| 15:00 | Squires Gate v Barnton | The Royal British Legion Stadium | |
| 15:00 | West Didsbury & Chorlton v 1874 Northwich | The Recreation Ground | |
| 15:00 | Winsford United v Barnoldswick Town | St Luke`s Barton Stadium | |
| Saturday 15th October 2016 | First Division | ||
| 15:00 | Ashton Town v Litherland REMYCA | The Ashton Town Stadium | |
| 15:00 | Atherton LR v Cheadle Town | Crilly Park | |
| 15:00 | Bacup Borough v Sandbach United | Brian Boys West View Stadium | |
| 15:00 | Chadderton v Holker Old Boys | ARK Fleetech Stadium | |
| 15:00 | Charnock Richard v Eccleshall | Mossie Park | |
| 15:00 | Daisy Hill v Widnes | New Sirs | |
| 15:00 | Prestwich Heys v City of Liverpool FC | Adie Moran Park | |
| 15:00 | Silsden v FC Oswestry Town | Keighley Road Stadium | |
| 15:00 | St Helens Town v Carlisle City | Valerie Park | |
| Played at Prescot Cables | |||
| 15:00 | Stockport Town v Alsager Town | Lambeth Grove | |
| 15:00 | Whitchurch Alport v AFC Blackpool | Yockings Park | |
| Monday 17th October 2016 | First Division | ||
| 19:45 | Daisy Hill v Stockport Town | New Sirs | |
| Monday 26th September 2016 Premier Division |
| Maine Road v Bootle |
| Tuesday 27th September 2016 Premier Division |
| 1874 Northwich v AFC Liverpool |
| Abbey Hey v Barnton |
| Ashton Athletic v Winsford United |
| Barnoldswick Tn v Irlam |
| Cammell Laird v Congleton Town |
| Tuesday 27th September 2016 First Division |
| Chadderton v Silsden |
| City of Lpool v Whitchurch Alp |
| Stockport Town v Lith REMYCA (20:00) |
| Tuesday 27th September 2016 Derbyshire Senior Cup 1st Rnd |
| Shirebrook Town v New Mills |
| Tuesday 27th September 2016 Lancashire Challenge Trophy 1st Rnd |
| Bamber Bridge v Charnock Rich |
| Tuesday 27th September 2016 Manchester Premier Cup 1st Rnd |
| Radcliffe Boro v West Did & Chor |
| Wednesday 28th September 2016 Premier Division |
| Runcorn Town v Hanley Town |
| Wednesday 28th September 2016 First Division |
| Cheadle Town v Widnes |
| Wednesday 28th September 2016 Lancashire Challenge Trophy 1st Rnd |
| Bacup Borough v Padiham (20:00) |
| Prestwich Heys v Chorley |
| Friday 30th September 2016 Premier Division |
| AFC Liverpool v Cammell Laird |
| Saturday 1st October 2016 The Macron Cup 1st Rnd |
|
| Barnton v Nelson | |
| Carlisle City v Ashton Athletic | |
| Congleton Town v Prestwich Heys | |
| FC Oswestry v Irlam | |
| Padiham v 1874 Northwich | |
| Runcorn Linnets v Daisy Hill | |
| Sandbach v Stockport Town | |
| Squires Gate v New Mills | |
| St Helens Town v AFC Darwen | |
| Played at Prescot Cables | |
| West Did & Chor v Barnoldswick Tn | |
| Widnes v Alsager Town | |
| Played at Alsager Town | |
| Winsford United v Atherton LR | |
| Saturday 1st October 2016 Premier Division |
| Abbey Hey v Hanley Town |
| Atherton Colls v Maine Road |
| Bootle v Runcorn Town |
| Saturday 1st October 2016 First Division |
| Ashton Town v Charnock Rich |
| Eccleshall v Bacup Borough |
| Holker OB v Cheadle Town |
| Lith REMYCA v AFC Blackpool |
| Silsden v City of Lpool |
| Whitchurch Alp v Chadderton |
| Monday 3rd October 2016 First Division |
| Daisy Hill v Bacup Borough |
| Tuesday 4th October 2016 Premier Division |
| 1874 Northwich v Barnton |
| Ashton Athletic v Atherton Colls |
| Barnoldswick Tn v Padiham |
| Congleton Town v Winsford United |
| Hanley Town v New Mills |
| Runcorn Linnets v Bootle |
| West Did & Chor v Squires Gate |
| Tuesday 4th October 2016 First Division |
| AFC Blackpool v Charnock Rich |
| City of Lpool v FC Oswestry |
| Whitchurch Alp v Widnes |
| Tuesday 4th October 2016 Manchester Premier Cup 1st Rnd |
| Abbey Hey v Trafford |
| Chadderton v |
| Glossop NE v Maine Road |
| Irlam v |
| Wednesday 5th October 2016 Premier Division |
| AFC Darwen v Nelson |
| Runcorn Town v AFC Liverpool |
| Wednesday 5th October 2016 First Division |
| Cheadle Town v Alsager Town |
| Friday 7th October 2016 Premier Division |
| West Did & Chor v Irlam |
| Saturday 8th October 2016 The Reusch First Division Cup 1st Rnd |
| AFC Blackpool v Carlisle City |
| Bacup Borough v Chadderton |
| Eccleshall v Stockport Town |
| Holker OB v Ashton Town |
| Prestwich Heys v Lith REMYCA |
| Widnes v FC Oswestry |
| Saturday 8th October 2016 Premier Division |
| 1874 Northwich v Abbey Hey |
| AFC Darwen v Cammell Laird |
| Ashton Athletic v Congleton Town |
| Barnoldswick Tn v Bootle |
| Barnton v Padiham |
| Hanley Town v Squires Gate |
| Maine Road v Runcorn Linnets |
| Nelson v AFC Liverpool |
| New Mills v Atherton Colls |
| Runcorn Town v Winsford United |
| Saturday 8th October 2016 First Division |
| Alsager Town v St Helens Town |
| Atherton LR v Whitchurch Alp |
| Charnock Rich v Cheadle Town |
| City of Lpool v Daisy Hill |
| Sandbach v Silsden |
| Monday 10th October 2016 Premier Division |
| Atherton Colls v Nelson |
| Winsford United v Maine Road |
| Tuesday 11th October 2016 Premier Division |
| Ashton Athletic v West Did & Chor |
| Barnoldswick Tn v Squires Gate |
| Bootle v Barnton |
| Runcorn Linnets v Cammell Laird |
| Tuesday 11th October 2016 First Division |
| AFC Blackpool v St Helens Town |
| Ashton Town v Atherton LR |
| Carlisle City v Charnock Rich |
| Chadderton v Stockport Town |
| Sandbach v Whitchurch Alp |
| Wednesday 12th October 2016 Premier Division |
| AFC Liverpool v Abbey Hey |
| Padiham v AFC Darwen |
| Wednesday 12th October 2016 First Division |
| Lith REMYCA v City of Lpool |
| Saturday 15th October 2016 Premier Division |
| Abbey Hey v Nelson |
| AFC Liverpool v New Mills |
| Atherton Colls v Irlam |
| Bootle v Hanley Town |
| Cammell Laird v Maine Road |
| Congleton Town v AFC Darwen |
| Padiham v Runcorn Linnets |
| Runcorn Town v Ashton Athletic |
| Squires Gate v Barnton |
| West Did & Chor v 1874 Northwich |
| Winsford United v Barnoldswick Tn |
| Saturday 15th October 2016 First Division |
|
| Ashton Town v Lith REMYCA | |
| Atherton LR v Cheadle Town | |
| Bacup Borough v Sandbach | |
| Chadderton v Holker OB | |
| Charnock Rich v Eccleshall | |
| Daisy Hill v Widnes | |
| Prestwich Heys v City of Lpool | |
| Silsden v FC Oswestry | |
| St Helens Town v Carlisle City | |
| Played at Prescot Cables | |
| Stockport Town v Alsager Town | |
| Whitchurch Alp v AFC Blackpool | |
| Monday 17th October 2016 First Division |
| Daisy Hill v Stockport Town |
